Part:BBa_K3610048:Design

CORE ectodomain / mCherry C-terminal for S. cerevisiae

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 1740
  Illegal BamHI site found at 373
  Illegal BamHI site found at 1765
  Illegal BamHI site found at 2117
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

mCherry part is codon optimized for expression in S. cerevisiae.

Flag tag fused to the mCherry sequence for western blotting

Source

BBa_K2809002, BBa_K3610010, BBa_K1150016, BBa_K3610019, BBa_M10019

References

Wang, Lei; Albert, Markus; Einig, Elias; Fürst, Ursula; Krust, Damaris; Felix, Georg (2016): The pattern-recognition receptor CORE of Solanaceae detects bacterial cold-shock protein. In: Nature plants 2, S. 16185. DOI: 10.1038/nplants.2016.185.

Fan, Jin-Yu; Cui, Zong-Qiang; Wei, Hong-Ping; Zhang, Zhi-Ping; Zhou, Ya-Feng; Wang, Yun-Peng; Zhang, Xian-En (2008): Split mCherry as a new red bimolecular fluorescence complementation system for visualizing protein–protein interactions in living cells. In: Biochemical and Biophysical Research Communications 367 (1), S. 47–53. DOI: 10.1016/j.bbrc.2007.12.101.